The injury suffered by Dilan Markanday in the 2-1 FA Cup defeat to Doncaster Rovers is not looking promising.

The attacker, who has just hit a good patch of form, pulled up in the second-half with a hamstring injury and he could not continue.

First-team coach Gary Roberts said: “Dilan doesn’t look too good. It looks like a hamstring again. Not ideal. We will scan him on Monday but it doesn’t look too clever.”

In other team news, Ryan Stirk returned from injury and got 55 minutes under his belt before coming off with cramp, while Will Grigg also ticked off 20 minutes on his comeback.

The game hinged on a straight red card for John Fleck, who had only been on the pitch six minutes when he was given his marching orders after a tackle on Owen Bailey. The Blues say they will review it and see if they can appeal it.

Roberts explained: “I think the game has changed on the decision. We had to go to a back five. I have not seen it back so I can’t really comment too much on the tackle but he (referee Neil Hair) seemed quite confident that it was a red card. John said that he had mistimed it but he didn’t think it was bad and that it was a yellow card. We will watch it back and see whether we can appeal.”

It was a lively cup tie overall which Chesterfield led through Lee Bonis after half an hour but Owen Bailey headed a reply three minutes later. The Spireites looked fairly comfortable despite being down to 10-men but they came undone in the 89th minute when substitute Jack Senior beat Zach Hemming at his near post to send Rovers through to round two.

Roberts added: “I thought when it was 11v11 we were the better team. I thought we were never really in trouble. We felt quite comfortable in the game. I thought we started the second-half properly, we highlighted that to the boys after Swindon last week. I thought it was one of our best performances for a while. Zach will be disappointed with the goal, for a keeper of his calibre, he saves those shots.”