Ryan Boot saved a penalty from Omari Patrick after Kyle McFadzean was punished for a foul and the hosts made the visitors pay just before half-time when Tom Naylor headed in James Berry’s inviting cross.
But Sam Finley equalised on 53 minutes, applying a first-time finish from inside the area, which was Tranmere’s first goal in six matches.
Naylor went close with two more headers late on but the Spireites did not do enough to deserve all three points against a Tranmere side who had lost nine of their previous 10 games.
The result means Chesterfield stay seventh in League Two.
Here are our player ratings from the game...
5. Malik Owolabi-Belewu 5
Malik Owolabi-Belewu 5 An early error presented the visitors with a gilt-edged chance which was wasted. Recovered okay but Blues maybe needed more from him going forward and he was replaced at half-time. Photo: Brian Eyre
6. Sammy Braybrooke 6
Sammy Braybrooke 6 Kept the ball ticking over in the middle of the park, made interceptions and tried to create. Influence softened after the break. Photo: Brian Eyre
7. Tom Naylor 7
Tom Naylor 7 Headed Town in front just before HT with one of his trademark runs into the box. Had a couple of late headers, one saved and one just wide. Photo: Tina Jenner
8. Dilan Markanday 6
Dilan Markanday 6 Ball looked to be stuck to his foot with glue times. Mixed up his game, linking play inside and providing width. Quieter after HT, off on 75. Photo: Brian Eyre