The Spireites will be underdogs at the Lamex Stadium but they have beaten Portsmouth and run West Brom and Watford close in the competition in recent years.
The hosts are fourth in League One, are unbeaten at home and have the best defensive record in the division so it will be a tough test for the visitors.
Let’s take a look at how Paul Cook’s men could line-up...
1. Zach Hemming - GK
Although it is a cup game we think that Hemming will keep his place between the sticks and that Ryan Boot will play in the upcoming EFL Trophy game against Liverpool's youths. Photo: Tina Jenner
2. Vontae Daley-Campbell - RB
This is a difficult one to call because Daley-Campbell has been in good form this season, but there are also two other players in Devan Tanton and Janoi Donacien who could probably do with some minutes and a cup game might be the ideal opportunity to do so. Photo: Tina Jenner
3. Chey Dunkley - CB
Donacien might be better suited to one of the centre-back positions rather than at right-back but Paul Cook may want to persist wih his main two central defenders for this one. Photo: Chesterfield FC
4. Kyle McFadzean - CB
He has ironed out some of his mistakes from previous weeks and is now looking like the centre-back which Chesterfield thought they were getting. Photo: Chesterfield FC