The Spireites have beaten Dorking Wanderers and AFC Fylde so far, scoring four goals in both matches.
The Hoops, meanwhile, are playing at this level for the first time in their history and have lost 5-2 at Aldershot Town and 1-0 at home to Rochdale.
Let’s take a look at how Town could line-up on Tuesday night...
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Chesterfield are in action away at Oxford City on Tuesday night. Picture: Tina Jenner. Photo: Tina Jenner
. Harry Tyrer - GK
Already a fans' favourite. Made a string of good saves on Saturday. This fixture presents a decent opportunity to get his first clean sheet. Photo: Alex Livesey
. Ryheem Sheckleford - RB
Some fans are calling for Jeff King to return at right-back but it's two wins from two and Paul Cook doesn't tend to change a winning team. Photo: Tina Jenner
. Ash Palmer - CB
Tyrone Williams was forced off at half-time on Saturday with a hamstring problem and Danny Webb said he is a doubt for Tuesday night. Palmer replaced him so you would think he will come in again if needed. Photo: Tina Jenner
. Jamie Grimes - CB
He was immense against AFC Fylde, in my opinion, and was a man of the match contender. Photo: Tina Jenner
. Branden Horton - LB
He was better against AFC Fylde and played a part in the second goal. Fellow left-back Bailey Clements is set to resume light training this week. Photo: Alex Livesey
1. Harry Tyrer - GK
Already a fans' favourite. Made a string of good saves on Saturday. This fixture presents a decent opportunity to get his first clean sheet. Photo: Alex Livesey
2. Ryheem Sheckleford - RB
Some fans are calling for Jeff King to return at right-back but it's two wins from two and Paul Cook doesn't tend to change a winning team. Photo: Tina Jenner
3. Ash Palmer - CB
Tyrone Williams was forced off at half-time on Saturday with a hamstring problem and Danny Webb said he is a doubt for Tuesday night. Palmer replaced him so you would think he will come in again if needed. Photo: Tina Jenner
4. Jamie Grimes - CB
He was immense against AFC Fylde, in my opinion, and was a man of the match contender. Photo: Tina Jenner