The Spireites could edge closer to the top seven with all three points.
The Shots, currently 13th in the National League, drew 3-3 with Boreham Wood on Saturday and will go above the Blues with a victory.
The reverse fixture finished goalless, but Chesterfield were denied by a number of top saves.
Here is Liam Norcliffe’s predicted Chesterfield line-up for the trip to The EBB Stadium...
1. Ross Fitzsimons - GK
The 28-year-old has re-signed for the Spireites and will make his second debut for the club. Photo: Pete Norton
2. Jeff King - RB
Continued his good pre-season form with an impressive outing against Dorking last weekend. Photo: Nathan Stirk
3. Tyrone Williams - RCB
The centre-back put in a solid display at Dorking and has impressed many with his performance in pre-season. Photo: Nathan Stirk
4. Jamie Grimes - CB
Captained the side on the opening day. Photo: Justin Setterfield