The Spireites are sixth in League Two and are eight unbeaten, while the Hornets are eighth in the Isthmian Premier Division and are also eight without a loss.
Town are likely to give players returning from injury some minutes but it will still be a strong line-up as they aim to continue their good record in this competition in the last few years.
Let’s take a look at how they could start...
1. Ryan Boot - GK
He has missed the last four matches with a wrist injury but he was expected to return to training this week so he could come back in all being well. Photo: Cameron Smith
2. Devan Tanton - RB
The right-back has been out injured for a month - last playing against Doncaster Rovers - but he is another one who was set to start training properly again this week. Photo: Tina Jenner
3. Chey Dunkley - CB
The dominant centre-back has been unavailable for the last four outings because of a neck injury but if he gets good news on another scan this week then he could also return. Williams could start if not. Photo: Tina Jenner
4. Jamie Grimes - CB
The skipper got 45 minutes - and a goal - in the second-half against Morecambe last weekend. He's had a frustrating start to the season minutes-wise but he could get a runout. Photo: Catherine Ivill