The decorated ducks have been sculptured and hand painted by artists from the borough after the final designs were chosen by the public in November.
The 1.5m tall fibreglass birds have been positioned in secret locations in the four market towns – Alfreton, Belper, Ripley and Heanor – with smaller ducks hidden in shop windows.
Trail maps are available, alongside a mobile app where visitors can collect all the ducks in one town or challenge themselves to visit all four towns.
Amber Valley Borough Council said the aim of the trail is to boost footfall in the town centres and encourage people to explore with well-loved local phrase Ay Up Me Duck chosen as the theme.
The trail runs for 10 weeks.

1. Ay up me duck trail
Artist Carla D Matthews and Leadre of Amber Valley Borough Council, Cllr Chris Emmas-Willams in Belper Market Place. Photo: Brian Eyre

2. Ay up me duck trail
Artist Carla D Matthews in Belper Market Place with the duck she designed and painted. Photo: Brian Eyre