Chesterfield travel to Rochdale on Saturday looking to bounce back from defeat at Scunthorpe United on Tuesday night.
A much-changed Spireites to the one that lost agonisingly at home to Leyton Orient battled hard but were undone by two second half goals.
Full debuts were handed to on loan defender Ashley Carter and winger Gboly Ariyibi for Chesterfield’s third trip to Glanford Park this season.
Sam Hird, Ritchie Humphreys and Byron Harrison were also drafted back in but goals from Niall Canavan and Paddy Madden won it for Iron.
It meant a third consecutive defeat for Chesterfield, who have slipped out of the League One play-off spots ahead of the trip to Spotland.
“We’re going through a little bit of a transitional period,” said manager Paul Cook.
“We’re having a little bit of a blip that has come off the back of the Preston game and we must get ourselves out of it.
“Our boys have been terrific for a long, long time and they need help at the minute.
“They don’t need people on their backs they need a pat on the back to lift ourselves up for the rest of the season.”
Hird returned following suspension and partnered Carter at the heart of defence in the absence of club captain Ian Evatt through injury.
Evatt sustained a concussion in time added on against Orient and will be monitored ahead of Rochdale, along with Gary Roberts (hamstring) and Dan Gardner (ankle).