Chesterfield will play host to Hull City in a pre-season friendly on Tuesday, July 21 (7.30pm).
Hull, managed by Steve Bruce, suffered relegation from the Premier League on the last day of the 2014/15 season.
It is the first fixture announced to be played at the Proact Stadium this summer with all others so far away.
The Spireites travel to Matlock Town on July 14 (7.45pm) before going to Ilkeston a fortnight later on July 28 (7.45pm).
A Chesterfield XI will go to Belper Town on July 29 (7pm) and Hartlepool United on August 1 (3pm).