Spireites head down the M1 to Milton Keynes for the third time this season.
Paul Cook’s side beat the Dons 1-0 in the FA Cup but it was deemed defender George Margreitter was ineligible.
Chesterfield returned as ordered by the authorities and won 1-0 again, so a hat-trick of wins will be the seventh-placed side’s main aim.
The Chesterfield’s boss will be sweating on the fitness of left-back Dan Jones and midfielder Ollie Banks.
Jones hopped off after a 10th-minute ankle injury in the 3-0 home win over Fleetwood Town while Banks missed the final few seconds of the victory with a dead leg.
Jones, even if fit, could miss out as his replacement off the bench, Gboly Ariyibi, set up two of the goals on Saturday, for Caolan Lavery and Sam Morsy, as he played wide midfield with Sam Clucas dropping to the back four.
Banks returned to top form in the match and will be a miss if he’s not fit, but the latest views are that he should make the trip.
Dan Gardner has returned to full training this week but he’s most likely to return to the squad for Chesterfield’s home match with Coventry City a week on Saturday.
Lavery will continue up top having scored the key opening goal and looking at his sharpest while Ian Evatt and Sam Hird, who combined for the second goal, are set to continue in the heart of the defence.
Karl Robinson’s Dons lost at Coventry at the weekend but remain in second spot.
Star midfielder Dele Alli is injured as is striker Dean Bowditch while keeper David Martin is doubtful.
Former Barnsley man Devante Cole and Will Grigg will be competing for a spot in attack.