A new state-of-the-art facility is set to open at Markham Vale - creating up to 30 jobs.
Sterigenics' bespoke sterilisation facility will officially open at the site in December.
The company specialises in sterilising medical equipment and has facilities in 13 countries including America and China. The new site will be in addition to its existing base at Somercotes.
Adrian Wye, director of operations of Sterigenics, said: "Our Markham Vale north facility is strategically located to serve the needs of our European customers.
"Its prime location at the heart of the UK's motorway network will provide an optimum location for their supply chain logistics.
"And our facility's high-tech capabilities will deliver the turnaround times necessary to distribute their products to market quickly."
Sterigenics will be the fourth business to join Gist, Ferdinand Bilstein UK and Great Bear Distribution on Markham Vale north.
Markham Vale, which is located just off junction 29a of the M1, is Derbyshire County Council and Henry Boot Development's flagship regeneration site.
Councillor Tony King, the council's cabinet member for economic development and regeneration, said: "We're delighted to welcome Sterigenics to our Markham Vale site and news they're set to create up to 30 jobs is fantastic.
"The firm's investment in Derbyshire shows how confident they are in the county as a place to grow their business and thrive.
"Markham's excellent location next to the M1 motorway is set to help the business reach more even more customers and we're looking forward to following the progress of their new state-of-the-art facility."
The company's new 69,000 sq ft base has been built by Henry Boot Developments - the council's private sector property partner.
Vivienne Clements, director of Henry Boot Developments, said: "We're pleased that Sterigenics has chosen Markham Vale for its UK base - it will no doubt prove an ideal location from which to serve and expand its European customer base.
"Generally, demand for well-located industrial and warehousing space has remained robust.
"At Markham Vale, we've worked hard to make sure we're not only offering direct motorway access in a prime central location but also the very best quality in terms of premises and service.
"Given the consistently high interest in remaining opportunities, it's a strategy that has paid off."
Businesses interested in acquiring premises at the site can contact Peter Storey, head of Markham Vale, on 01629 535417 for more information or visit ww.mawrkhamvale.co.uk